functional motifs in escherichia coli nc101

Functional motifs in Escherichia coli NC101

Escherichia coli (E. coli) bacteria can damage DNA of the gut lining cells and may encourage the development of colon cancer according to recent reports. Genetic switches are specific sequence motifs and many of them are drug targets. It is interesting to know motifs and their location in sequences. At the present study, Gibbs sampler algorithm was used in order to predict and find functional m...

Functional motifs in Escherichia coli NC101

Escherichia coli (E. coli) bacteria can damage DNA of the gut lining cells and may encourage the development of colon cancer according to recent reports. Genetic switches are specific sequence motifs and many of them are drug targets. It is interesting to know motifs and their location in sequences. At the present study, Gibbs sampler algorithm was used in order to predict and find functional m...

The determinants of transcriptional network motifs assembly in Escherichia coli

We assembled a transcriptional regulatory network (TRN) with data from Escherichia coli’s RegulonDB (v.5.6) [1]. In this network, each interaction is given by the operon encoding the transcriptional factor (TF), that encoding the target gene, and a directional link (edge) representing the transcriptional regulation, being this positive, negative or dual. The TRN is constituted by 681 nodes and ...

Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions

The RadA/Sms protein is a RecA-related protein found universally in eubacteria and plants, implicated in processing of recombination intermediates. Here we show that the putative Zn finger, Walker A motif, KNRXG motif and Lon protease homology domain of the Escherichia coli RadA protein are required for DNA damage survival. RadA is unlikely to possess protease activity as the putative active si...

cloning and expression of functional reteplase in escherichia coli top10

background: production of tissue plasminogen activator protein (t-pa) in prokaryotes systems has many problems such as the lack of active protein production, multiple purification steps, and renaturation process which has been shown to be costly and time-consuming. methods: in this study, reteplase which is the nonglycosylated active domain of t-pa was used to transform top10 escherichia coli (...

Advances and challenges in recombinant protein expression in Escherichia coli